Overview
Type: Synthetic peptide
Form: Lyophilized powder
Trace Amine Receptor 1/TAAR1 Blocking Peptide (#BLP-TR021) is the original antigen used for immunization during Anti-Trace Amine Receptor 1 (TAAR1) Antibody (#ATR-021) generation. The blocking peptide binds and ‘blocks’ Anti-Trace Amine Receptor 1/TAAR1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat liver (lane 1 and 4), mouse kidney (lanes 2 and 5) and human brain glioblastoma (U-87) cell (lanes 3 and 6) lysates:1-3. Anti-Trace Amine Receptor 1 (TAAR1) Antibody (#ATR-021), (1:200).
4-6. Anti-Trace Amine Receptor 1 (TAAR1) Antibody, preincubated with Trace Amine Receptor 1/TAAR1 Blocking Peptide (#BLP-TR021).
Properties
Sequence
- (C)KMVLFGKIFQKDS, corresponding to amino acid residues 313-325 of rat TAAR1 (Accession Q923Y9).
Accession (Uniprot) Number Q923Y9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
