TREM2 (extracellular) Blocking Peptide (#BLP-NR018) is the original antigen used for immunization during Anti-TREM2 (extracellular) Antibody (#ANR-018) generation. The blocking peptide binds and ‘blocks’ Anti-TREM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Western blot analysis of mouse BV-2 microglia (lanes 1 and 4), human U-87 MG glioblastoma (lanes 2 and 5) and human THP-1 monocytic leukemia (lanes 3 and 6) cell line lysates:
- Expression of TREM2 in mouse hippocampus in a kainic acid neurodegeneration model.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections collected 4 days post kainate injection with Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region showed TREM2 immunoreactivity (green) in astrocytes in the stratum oriens (SO) and the stratum radiatum (SR). B. Pre-incubation of the antibody with TREM2 (extracellular) Blocking Peptide (BLP-NR018), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)KHWGRRKAW(S)RQLGE, corresponding to amino acid residues 42-56 of mouse TREM2 (Accession Q99NH8).