Overview
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-TrkA (extracellular) Antibody (#ANT-018) and Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (#ANT-018-FR). Intended to be used as a negative control.
Applications: bl
For research purposes only, not for human use
Applications
Applications
Blocking
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-TrkA (extracellular) Antibody (#ANT-018), (1:200).
3,4. Anti-TrkA (extracellular) Antibody, preincubated with TrkA (extracellular) Blocking Peptide (#BLP-NT018).
Expression of TrkA in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkA (extracellular) Antibody (#ANT-018), (1:100). TrkA (red staining) is expressed in DRG neurons and in satellite microglia (arrows). Hoechst 33342 is used as the counterstain.
Expression of TrkA in rat brain hippocampal dentate gyrusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections. A. Brain sections were stained using Anti-TrkA (extracellular) Antibody (#ANT-018), (1:1000), (green staining). B. The same section was also stained for glial fibrillary acidic protein (GFAP) (red and counterstained blue). C. Overlay of A and B demonstrates co-localization of TrkA and GFAP in dentate gyrus astrocytes.
Expression of TrkA in rat dorsal root ganglionImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (#ANT-018-FR), (1:50). TrkA (purple staining) is expressed in DRG neurons. Hoechst 33342 (blue) is used as the counterstain.
Immunolocalization of p75NTR and TrkA in rat dorsal root ganglionImmunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody (#ANT-007-AG), (1:60) and Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (#ANT-018-FR), (1:60). A. Sections were labeled with Anti-p75 NGR Receptor (extracellular)-ATTO Fluor-488 Antibody (green). B. The same section was stained with Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (purple). C. Merge of the two images suggests extensive co-localization (arrow points at an example) of TrkA and p75NTR receptors in rat dorsal root ganglion. Cell nuclei were stained with DAPI (blue).
Properties
Properties
Sequence
- (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA (Accession P35739).
Accession (Uniprot) Number P35739
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Shipping and Ordering information
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