Overview
Cat #: BLP-NT018
Type: Synthetic peptide
Form: Lyophilized powder
TrkA (extracellular) Blocking Peptide (#BLP-NT018) is the original antigen used for immunization during Anti-TrkA (extracellular) Antibody (#ANT-018) generation. The blocking peptide binds and ‘blocks’ Anti-TrkA (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-TrkA (extracellular) Antibody (#ANT-018), (1:200).
3,4. Anti-TrkA (extracellular) Antibody, preincubated with TrkA (extracellular) Blocking Peptide (#BLP-NT018). - Expression of TrkA in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkA (extracellular) Antibody (#ANT-018), (1:100). TrkA (red staining) is expressed in DRG neurons and in satellite microglia (arrows). Hoechst 33342 is used as the counterstain.
- Expression of TrkA in rat brain hippocampal dentate gyrusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections. A. Brain sections were stained using Anti-TrkA (extracellular) Antibody (#ANT-018), (1:1000), (green staining). B. The same section was also stained for glial fibrillary acidic protein (GFAP) (red and counterstained blue). C. Overlay of A and B demonstrates co-localization of TrkA and GFAP in dentate gyrus astrocytes.
Properties
Sequence
Accession (Uniprot) Number P35739
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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