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Certificate of Analysis

TrkB (extracellular) Blocking Peptide

Overview

Cat #: BLP-NT019
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: bl

Application key:

BL- Blocking
For research purposes only, not for human use

Applications

Applications

Blocking
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and HEK-TrkB transfected cell lysates (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and HEK-TrkB transfected cell lysates (lanes 2 and 4):
    1,2. Anti-TrkB (extracellular) Antibody (#ANT-019), (1:200).
    3,4. Anti-TrkB (extracellular) Antibody, preincubated with TrkB (extracellular) Blocking Peptide (#BLP-NT019).
  • Western blot analysis of mouse brain lysates:
    Western blot analysis of mouse brain lysates:
    1. Anti-TrkB (extracellular) Antibody (#ANT-019), (1:200).
    2. Anti-TrkB (extracellular) Antibody, preincubated with TrkB (extracellular) Blocking Peptide (#BLP-NT019).
  • Rat dorsal root ganglion sections (1:100), (frozen).
  • Rat DRG sections.
  • Expression of TrkB in rat DRG
    Expression of TrkB in rat DRG
    Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkB (extracellular)-ATTO-488 Antibody (#ANT-019-AG), (1:50), (green). Staining is present in DRG neurons. Hoechst 33342 is used as the counterstain (blue).

Properties

Properties

Sequence
  • (C)RLEPNSIDPENITE, corresponding to amino acid residues 57-70 of rat TrkB (Accession Q63604).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
Last update: 23/08/2020
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