TrkB (extracellular) Blocking Peptide (#BLP-NT019) is the original antigen used for immunization during Anti-TrkB (extracellular) Antibody (#ANT-019) generation. The blocking peptide binds and ‘blocks’ Anti-TrkB (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and HEK-TrkB transfected cell lysates (lanes 2 and 4):
- Western blot analysis of mouse brain lysates:
- Expression of TrkB in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkB (extracellular) Antibody (#ANT-019), (1:100). TrkB (red) is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
- (C)RLEPNSIDPENITE, corresponding to amino acid residues 57-70 of rat TrkB (Accession Q63604).