TROY/TNFRSF19 (extracellular) Blocking Peptide (#BLP-NT033) is the original antigen used for immunization during Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033) generation. The blocking peptide binds and ‘blocks’ Anti-TROY/TNFRSF19 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4):
- Western blot analysis of new born rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- CRPHRFKEDWGFQK, corresponding to amino acid residues 75-88 of mouse TROY (Accession Q9JLL3).