TRPP1/PKD2 Blocking Peptide (#BLP-CC052) is the original antigen used for immunization during Anti-TRPP1 (PKD2) Antibody (#ACC-052) generation. The blocking peptide binds and ‘blocks’ Anti-TRPP1/PKD2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney membrane proteins (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4):
- Expression of TRPP1 (PKD2) in rat kidneyImmunohistochemical staining of rat kidney paraffin embedded sections using Anti-TRPP1 (PKD2) Antibody (#ACC-052), (1:100), followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody. A. TRPP1 staining followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody (red) appears in the renal cortex, mainly in the distal tubules. B. DAB staining of TRPP1 (brown) also shows that the renal corpuscle and the blood vessels are not stained at all. (PT = proximal tubule, DT = distal tubule).
- (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 of human TRPP1 (Accession Q13563).