Cat #: BLP-CC052
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-TRPP1 (PKD2) Antibody (#ACC-052). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat kidney membrane proteins (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4):
- Expression of TRPP1 (PKD2) in rat kidneyImmunohistochemical staining of rat kidney paraffin embedded sections using Anti-TRPP1 (PKD2) Antibody (#ACC-052), (1:100), followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody. A. TRPP1 staining followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody (red) appears in the renal cortex, mainly in the distal tubules. B. DAB staining of TRPP1 (brown) also shows that the renal corpuscle and the blood vessels are not stained at all. (PT = proximal tubule, DT = distal tubule).
- (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 of human TRPP1 (Accession Q13563).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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