Overview
Cat #: BLP-CC033
Type: Synthetic peptide
Form: Lyophilized powder
TRPV3 (extracellular) Blocking Peptide (#BLP-CC033) is the original antigen used for immunization during Anti-TRPV3 (extracellular) Antibody (#ACC-033) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat DRG (lanes 1 and 2) and brain (lanes 3 and 4) lysates:1,3. Anti-TRPV3 (extracellular) Antibody (#ACC-033), (1:200).
2,4. Anti-TRPV3 (extracellular) Antibody, preincubated with TRPV3 (extracellular) Blocking Peptide (#BLP-CC033). - Expression of TRPV3 in mouse spinal cordImmunohistochemical staining of mouse spinal cord using Anti-TRPV3 (extracellular) Antibody (#ACC-033). A. TRPV3 (green) appears in neurons (vertical arrows) in the ventral horn of the mouse spinal cord. B. Motor neurons were stained with goat anti-choline-acetyltransferase (red). In some motor neurons (A) TRPV3 was more intense (vertical arrow) whereas in others it was weak (horizontal arrows). C. Merged images of panels A and B. The inset in C magnifies one large motor neuron (vertical arrowhead). Note the punctate pattern of TRPV3 on the surface of the soma.
Properties
Sequence
Accession (Uniprot) Number Q8NET8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.