Overview
Type: Synthetic peptide
Form: Lyophilized powder
Vasopressin V1B Receptor/AVPR1B Blocking Peptide (#BLP-VR011) is the original antigen used for immunization during Anti-Vasopressin V1B Receptor (AVPR1B) Antibody (#AVR-011) generation. The blocking peptide binds and ‘blocks’ Anti-Vasopressin V1B Receptor/AVPR1B primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 3) and mouse kidney (lanes 2 and 4) lysates:1,2. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody (#AVR-011), (1:200).
3,4. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody, preincubated with Vasopressin V1B Receptor/AVPR1B Blocking Peptide (#BLP-VR011).
Properties
Sequence
- (C)RAKPRPAGSLKDLE, corresponding to amino acid residues 399-412 of rat AVPR1B (Accession P48974).
Accession (Uniprot) Number P48974
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
