Overview
Type: Synthetic peptide
Form: Lyophilized powder
Vesicular Acetylcholine Transporter/VACHT Blocking Peptide (#BLP-CT003) is the original antigen used for immunization during Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody (#ACT-003) generation. The blocking peptide binds and ‘blocks’ Anti-Vesicular Acetylcholine Transporter/VACHT primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody (#ACT-003), (1:200).
3,4. Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody, preincubated with Vesicular Acetylcholine Transporter/VACHT Blocking Peptide (#BLP-CT003).
Expression of VAChT in rat medial septum and spinal cordImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody (#ACT-003), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. A. VAChT staining (green) in rat medial septum is detected in neuronal soma (arrows). B. Staining of spinal cord sections reveals VAChT labeling in neuronal soma (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DAVRLREVQGKDGGE, corresponding to amino acid residues 495-509 of rat VAChT (Accession Q62666).
Accession (Uniprot) Number Q62666
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
