Overview
Type: Synthetic peptide
Form: Lyophilized powder
xCT/SLC7A11 (extracellular) Blocking Peptide (#BLP-NT111) is the original antigen used for immunization during Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111) generation. The blocking peptide binds and ‘blocks’ Anti-xCT/SLC7A11 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat stomach (lanes 3 and 6) lysates:1-3. Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111), (1:800).
4-6. Anti-xCT/SLC7A11 (extracellular) Antibody, preincubated with xCT/SLC7A11 (extracellular) Blocking Peptide (#BLP-NT111).
Western blot analysis of human brain glioblastoma cell line (U-87 MG) lysate:1. Anti-xCT/SLC7A11 (extracellular) Antibody (1:200).
2. Anti-xCT/SLC7A11 (extracellular) Antibody, preincubated with xCT/SLC7A11 (extracellular) Blocking Peptide (#BLP-NT111).
Expression of xCT/SLC7A11 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SLC7A11 staining in hippocampal CA1 region is detected in the pyramidal layer (P, vertical arrows) and in glial profiles in stratum radiatum (SR, horizontal arrows). B. Pre-incubation of the antibody with xCT/SLC7A11 (extracellular) Blocking Peptide (#BLP-NT111), suppresses staining. Cell nuclei are stained with DAPI (blue). P = Pyramidal layer; SR = Stratum radiatum; SO = Stratum oriens.
Properties
Sequence
- (C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse cystine/glutamate transporter (Accession Q9WTR6).
Accession (Uniprot) Number Q9WTR6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
