ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003) is the original antigen used for immunization during Anti-ZIP3 (SLC39A3) Antibody (#AZT-003) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP3/SLC39A3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse lung (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
- Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell lysate (lanes 2 and 4):
- Expression of Zinc transporter ZIP3 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 antibody. ZIP3 staining (green) appears in CA1 neurons in the pyramidal layer (arrow). Nuclei are labeled with DAPI (blue).
- (C)EADFEKAHRSKK, corresponding to amino acid residues 31-42 of rat ZIP3 (Accession Q5U1X7).