Overview
Cat #: BLP-ZT008
Type: Synthetic peptide
Form: Lyophilized powder
ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008) is the original antigen used for immunization during Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP8/SLC39A8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse kidney (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
3,4. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008). - Western blot analysis of rat brain lysate:1. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008). - Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 4) lysates:1,2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
3,4. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008). - Expression of Zinc transporter ZIP8 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ZIP8 staining (green) appears in interneurons (arrows) adjacent to the granular layer (GL). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
Accession (Uniprot) Number Q5FVQ0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.