ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008) is the original antigen used for immunization during Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP8/SLC39A8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse kidney (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
- Western blot analysis of rat brain lysate:
- Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 4) lysates:
- Expression of Zinc transporter ZIP8 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ZIP8 staining (green) appears in interneurons (arrows) adjacent to the granular layer (GL). Cell nuclei are stained with DAPI (blue).
- (C)KTYGQNDHTHFRND, corresponding to amino acid residues 221-234 of rat ZIP8 (Accession Q5FVQ0).