Overview
Type: Synthetic peptide
Form: Lyophilized Powder
ZnT3/SLC30A3 Blocking Peptide (#BLP-ZT013) is the original antigen used for immunization during Anti-ZnT3 (SLC30A3) Antibody (#AZT-013) generation. The blocking peptide binds and ‘blocks’ Anti-ZnT3/SLC30A3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-ZnT3 (SLC30A3) Antibody (#AZT-013), (1:500).
3,4. Anti-ZnT3 (SLC30A3) Antibody, preincubated with ZnT3/SLC30A3 Blocking Peptide (#BLP-ZT013).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1. Anti-ZnT3 (SLC30A3) Antibody (#AZT-013), (1:200).
2. Anti-ZnT3 (SLC30A3) Antibody, preincubated with ZnT3/SLC30A3 Blocking Peptide (#BLP-ZT013).
Expression of Zinc transporter 3 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-ZnT3 (SLC30A3) Antibody (#AZT-013), (1:600). A. ZnT3 (green) is expressed in the mossy fiber (MF) terminal field of the CA3 region rat hippocampal region. B. Synaptophysin staining (red) is detected in both the MF terminal field and the CA3 region. C. Merge of the two images reveals extensive co-localization (arrows) in the MF region. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RGAEYAPLEEGHGH, corresponding to amino acid residues 210-223 of rat ZnT3 (Accession Q6QIX3).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
