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Proton-Activated Chloride Channel 1 and Lyso-PS Receptors

New products are here for those of you working in neuro, immunology, and membrane protein fields.

It’s not every day there’s a new antibody against a new ion channel plus a whole new group of GPCR products, but today is one of those days.  

Proton-Activated Chloride Channels

You can now get a primary antibody against proton-activated Cl- channel 1 (PACC1), also known as TMEM206. This channel has been known for quite a while but was only described formally in 2019 when it was found to respond to extracellular acidic pH at which point it imports chloride ions (Yang et al., 2019Ullrich et al., 2019).

Our Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031) targets the extracellular epitope (peptide (C)KLKHPVMSVSYKEVDR, which corresponds to amino acid residues 95–110 of mouse PACC1) and we’ve tested it in western blot, immunohistochemistry, and flow cytometry. And as with everything, it was designed and made right here at Alomone Labs. 

Take a look at some of the data:

Figure 1: Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5), and rat kidney lysates (lanes 3 and 6): 1–3. Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200).  4–6. Anti-PACC1/TMEM206 (extracellular) Antibody, preincubated with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031).
Figure 2. Expression of PACC1 in mouse reticular thalamic nucleus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200), followed by goat anti-rabbit AlexaFluor-488. A. PACC1 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031), suppressed staining. Cell nuclei are stained with DAPI (blue).

Lysophosphatidylserine Receptors

Lysophosphatidylserines or Lsyo-PSs, are hydrolysis products phosphatidylserine (PS) and are gathering a lot of interest as a newer form of lysophospholipids with a role in hormone-like signaling. These Lyso-PSs also seem to specifically activate three human G protein-coupled receptors (GPCRs): GPR34/LPS1, P2Y10/LPS2, and GPR174/LPS3 (Sugo et al., 2006Inoue et al., 2012).

Given the sizable number of drugs that target GPCRS, the desire to identify GPCR ligands, and the link between Lyso-PS deregulation and human disease (all reviewed in Shanbhag et al., 2021), we thought Lyso-PS receptors deserved their own product category. 

And since these GPCRs are expressed in both the brain and immune system, those of you working in neuroscience or neuroinflammation might be especially interested in what we have:

GPR34/LPS1

Anti-GPR34 (extracellular) Antibody (#AGR-055) 

Anti-GPR34 (extracellular)-FITC Antibody (#AGR-055-F)

GPR34 (extracellular) Blocking Peptide (#BLP-GR055)

P2Y10/LPS2

Anti-P2Y10 (extracellular) Antibody (#APR-110) 

P2Y10 (extracellular) Blocking Peptide (# BLP-PR110)

GPR174/LPS3

Anti-GPR174 (extracellular) Antibody (#AGR-060)

Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F)

GPR174 (extracellular) Blocking Peptide (#BLP-GR060)


Something you need that isn’t on the catalog? There’s a good chance we can make it for you. Have a look at our Customer Services and get what your research needs.