Extracellular antibodies are ideal research tools for cell-surface proteins.
No permeabilization/fixation because using extracellular antibodies allows you to work with live cells!
Benefits of live cell imaging:
✓ Monitor cell movement
✓ Study protein transport across the cell
✓ Investigate protein internalization
NaV1.7 (Scn9a) is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain-of-function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities). Loss-of-function mutations in NaV1.7 channels, lead to complete ablation of pain perception in humans. Scn9a is a major therapeutic target for pain.