Anti-NaV1.7 (SCN9A) (extracellular) Antibody

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
    Cat #: ASC-027
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: r
    May also work in: m
    Immunogen
    Peptide (C)EKEGQTEYMDY(K), corresponding to amino acid residues 1533-1543 of rat NaV1.7 (Accession O08562). 1st extracellular loop, domain IV.
    Accession (Uniprot) Number O08562
    Gene ID 78956
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 8/11 amino acid residues identical.
    Human - Not recommended to use with human samples.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat brain (lanes 1 and 3) and rat DRG (lanes 2 and 4) lysates:
    1,2. Anti-NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-027), (1:200).
    3,4. Anti-NaV1.7 (SCN9A) (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of NaV1.7 in rat PC12 cells
    Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-027), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge image of A and live view of the cells.
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Fang, X. et al. (2002) J. Neurosci22, 7425.
    3. Fjell, J. et al. (2000) NeuroReport 11, 199.
    4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
    5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    6. Isom, L.L. (2001) Neuroscientist  7, 42.
    7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
    8. Catterall, W.A. et al. (2005) Pharmacol. Rev57, 397.
    9. Yang, Y. et al. (2004) J. Med. Genet. 41, 171.
    10. Cummins, T.R. et al. (2004) J. Neurosci. 24, 8232.
    11. Dray, A. (2008) Br. J. Anaesth101, 48.
    Scientific background

    Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.

    Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.

    NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat NaV1.7 channel. Anti-NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-027) can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and thus is ideal for detecting NaV1.7 in living cells. It has been designed to recognize NaV1.7 from rat and mouse samples. The antibody will not recognize NaV1.7 from human samples.

    For research purposes only, not for human use
    Citations
    Related Products
      • Products for control experiments
        1. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008). This antibody recognizes a different epitope and thus can also be used as a control for detecting NaV1.7.
      • Antibodies for immuno-colocalization experiments
        1. Anti-NaV1.7 (SCN9A)-ATTO-633 Antibody (#ASC-008-FR). A fluorescent labeled primary antibody. It can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.
        2. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#AGP-057) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #ASC-008.