α-Conotoxin AuIB

A Potent Antagonist of Mammalian α3/β4 Neuronal nAChR
    Cat #: STC-310
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1572 Da.
    Purity: >99% (HPLC)
    Effective concentration 0.1-5 μM.
    Sequence GCCSYPPCFATNPDC.
    Modifications Disulfide bonds between Cys2-Cys8 and Cys3-Cys15. Cys15 - C-terminal amidation.
    Structure
    • α-Conotoxin AuIB
    Molecular formula C65H89N17O21S4.
    CAS No.: 216299-21-7.
    Activity α-Conotoxin AuIB potently and specifically blocks mammalian neuronal nAChR channels α3/β41.
    References-Activity
    1. Luo, S. et al. (1998) J. Neurosci. 18, 8571.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • α-Conotoxin AuIB
      Alomone Labs α-Conotoxin AuIB inhibits α3/β4 nAChR channels heterologously expressed in Xenopus oocytes.
      Superimposed traces of α3/β4 nAChR channel current in the absence (black) and presence of different concentrations of α-Conotoxin AuIB (#STC-310). Current amplitudes were plotted as a function of time. Membrane potential was held at -60 mV and oocytes were stimulated by exposure to 20 μM Acetylcholine and 1 μM Atropine, for 1 second every 50 seconds. The indicated different α-Conotoxin AuIB concentrations (inset) were perfused during the period marked in colors.
    References - Scientific background
    1. Luo, S. et al. (1998) J. Neurosci. 18, 8571.
    2. Dutton, J.L. et al. (2002) J. of Biol. Chem. 277, 48849.
    Scientific background

    α-Conotoxin AuIB is a 15 amino acid peptidyl toxin isolated from Conus aulicus1. This toxin inhibits mammalian neuronal α3/β4 nicotinic acetylcholine receptor (nAChR) channels expressed in Xenopus oocytes with an IC50 of 0.75 µM. Furthermore, α-Conotoxin AuIB blocks the α3/β4 receptor with >100-fold higher potency than other receptor subunit combinations1.

    In accordance, α-Conotoxin AuIB (1-5 µM) blocks 20-35% of the nicotine-stimulated norepinephrine release from rat hippocampal synaptosomes1 and the nicotinic ACh receptors on dissociated neurons of the rat parasympathetic ganglia with IC50 of 1.2 nM2.

    Target α3/β4 nAChR
    Net Peptide Content: 100%
    Last update: 24/01/2020

    α-Conotoxin AuIB (#STC-310) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Shipping and Ordering information