Cat #: C-290
Alternative Name α-CTx ImI
This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
Origin Synthetic peptide
MW: 1351 Da.
Purity: >99% (HPLC)
Effective concentration 0.1-10 μM.
Modifications Disulfide bonds between Cys2-Cys8 and Cys3-Cys12. Cys12 - C-terminal amidation.
Molecular formula C52H78N20O15S4.
CAS No.: 156467-85-5.
Activity α-Conotoxin ImI potently and specifically blocks mammalian neuronal nAChR (α3/β2 > α7 > α3/β4)1.
- McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
- Alomone Labs α-Conotoxin ImI inhibits α3/β2 nAChR channels heterologously expressed in Xenopus oocytes.Time course of α-Conotoxin ImI (#C-290) action on α3/β2 nAChR channels. Current amplitudes were plotted as a function of time. Membrane potential was held at –80 mV and oocytes were stimulated by exposure to 10 nM acetylcholine and 10 μM atropine for 5 seconds every 100 seconds. The indicated α-CTx ImI concentrations (inset) were perfused during the period marked by color.
References - Scientific background
Scientific background α-Conotoxin ImI is a 12 amino acid peptidyl toxin isolated from Conus Imperialis venom1, which blocks mammalian neuronal nAChRs located on postsynaptic membranes. This toxin inhibits mammalian neuronal nAChRs (α3/β2 > α7 > α3/β4) in a voltage-independent manner and has no effect on nAChRs composed of α2/β2, α3/β2, α4/β2, α2/β4, α3/β4, or α4/β4 subunits1,2.
Target α3/β2 nAChR
Net Peptide Content: 100%
Last update: 24/01/2020
α-Conotoxin ImI (#C-290) is a highly pure, synthetic, and biologically active peptide toxin.
For research purposes only, not for human use
- Kiss, T. et al. (2014) PLoS ONE 9, e109538.
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