α-Conotoxin ImI

α-CTx ImI
A Potent Antagonist of Mammalian α3/β2 Neuronal nAChR
    Cat #: C-290
    Alternative Name α-CTx ImI
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1351 Da.
    Purity: >99% (HPLC)
    Effective concentration 0.1-10 μM.
    Sequence GCCSDPRCAWRC.
    Modifications Disulfide bonds between Cys2-Cys8 and Cys3-Cys12. Cys12 - C-terminal amidation.
    Structure
    • α-Conotoxin ImI
    Molecular formula C52H78N20O15S4.
    CAS No.: 156467-85-5.
    Activity α-Conotoxin ImI potently and specifically blocks mammalian neuronal nAChR (α3/β2 > α7 > α3/β4)1.
    References-Activity
    1. McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • α-Conotoxin ImI
      Alomone Labs α-Conotoxin ImI inhibits α3/β2 nAChR channels heterologously expressed in Xenopus oocytes.
      Time course of α-Conotoxin ImI (#C-290) action on α3/β2 nAChR channels. Current amplitudes were plotted as a function of time. Membrane potential was held at –80 mV and oocytes were stimulated by exposure to 10 nM acetylcholine and 10 μM atropine for 5 seconds every 100 seconds. The indicated α-CTx ImI concentrations (inset) were perfused during the period marked by color.
    References - Scientific background
    1. McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
    2. Johnson, D.S. et al. (1995) Mol. Pharmacol. 48, 194.
    Scientific background α-Conotoxin ImI is a 12 amino acid peptidyl toxin isolated from Conus Imperialis venom1, which blocks mammalian neuronal nAChRs located on postsynaptic membranes. This toxin inhibits mammalian neuronal nAChRs (α3/β2 > α7 > α3/β4) in a voltage-independent manner and has no effect on nAChRs composed of α2/β2, α3/β2, α4/β2, α2/β4, α3/β4, or α4/β4 subunits1,2.
    Target α3/β2 nAChR
    Net Peptide Content: 100%
    Last update: 24/01/2020

    α-Conotoxin ImI (#C-290) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Product citations
    1. Kiss, T. et al. (2014) PLoS ONE 9, e109538.
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