Overview
Cat #: BLP-SR021
Type: Synthetic peptide
Form: Lyophilized powder
5HT1A Receptor/HTR1A Blocking Peptide (#BLP-SR021) is the original antigen used for immunization during Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021) generation. The blocking peptide binds and ‘blocks’ Anti-5HT1A Receptor/HTR1A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021), (1:200).
3,4. Anti-5HT1A Receptor (HTR1A) Antibody, preincubated with 5HT1A Receptor/HTR1A Blocking Peptide (#BLP-SR021). - Expression of 5-Hydroxytryptamine receptor 1A in rat cerebellumImmunohistochemical staining of rat cerebellum frozen section using Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021), (1:100). 5-HT1A is expressed in Purkinje cells (red). Hoechst 33342 is used as the counterstain.
Properties
Sequence
Accession (Uniprot) Number P19327
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.