Cat #: BLP-SR031
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031), (1:200).
3,4. Anti-5HT3A Receptor (HTR3A) Antibody, preincubated with 5HT3A Receptor/HTR3A Blocking Peptide (#BLP-SR031).
- Expression of Serotonin receptor 3A in rat brainImmunohistochemical staining of rat parietal cortex using Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031), (1:400), (red). A. 5-HT3A staining appears in neuronal outlines (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
- (C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine receptor 3A (Accession P35563).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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