Anti-5HT3A Receptor (HTR3A) Antibody

5-Hydroxytryptamine receptor 3A, Serotonin receptor 3A, Serotonin-gated ion channel receptor 3A
    Cat #: ASR-031
    Alternative Name 5-Hydroxytryptamine receptor 3A, Serotonin receptor 3A, Serotonin-gated ion channel receptor 3A
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide (C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine receptor 3A (Accession P35563). 2nd intracellular loop.
        Anti-5HT3A Receptor (HTR3A) Antibody
    Accession (Uniprot) Number P35563
    Gene ID 79246
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/14 amino acid residues identical.
    RRID AB_10918023.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-5HT3A Receptor (HTR3A) Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
        1,2. Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031), (1:200).
        3,4. Anti-5HT3A Receptor (HTR3A) Antibody, preincubated with the control peptide antigen.
      • Anti-5HT3A Receptor (HTR3A) Antibody
        Expression of Serotonin receptor 3A in rat brain
        Immunohistochemical staining of rat parietal cortex using Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031), (1:400), (red). A. 5-HT3A staining appears in neuronal outlines (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
    References
      • 5-Hydroxytryptamine receptor 3A (5-HT3A, HTR3A) belongs to the superfamily of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the superfamily of G-protein coupled receptors.

        The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.

        In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The 5-HT3A subunit is expressed in GABAergic and enkephalinergic neurons in the spinal dorsal horn thereby marking its possible antinociceptive effect4.

        5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-5HT3A Receptor (HTR3A) Antibody
    Expression of metabotropic and ionotropic 5-HT Receptors in mouse trigeminal ganglion.
    Immunohistochemical staining of mouse trigeminal ganglion (TG) sections using Alomone Labs Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035) and Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031) (red) shows that all three receptors are expressed in retrogradely labeled whisker afferent neurons.Adapted from Chang, W. et al. (2016) with permission of the National Academy of Sciences, USA.
    Last update: 10/11/2019

    Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3A. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5-HT3A from mouse, human, and rat samples. 

    For research purposes only, not for human use