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Home › Products › Neural Signaling › Neurotransmission › Serotonin Signaling › 5-HT3 Receptors (Serotonin gated channels) › Antibodies to 5-HT3 Receptors › Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody › Blocking Peptide

Certificate of Analysis

5HT3B Receptor/HTR3B (extracellular) Blocking Peptide

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Cat #: BLP-SR032
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry
For research purposes only, not for human use

Properties

Sequence
  • (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B (Accession Q9JJ16).
Accession (Uniprot) Number Q9JJ16
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.

Applications

Demonstration of Pre-adsorption control
  • Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):
    Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):
    1,2. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032), (1:200).
    3,4. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody, preincubated with 5HT3B Receptor/HTR3B (extracellular) Blocking Peptide (#BLP-SR032).
  • Rat DRGs.
Last update: 08/01/2023

Specific Use it with:

  • Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3B. The antibody can be used in western blot  and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5-HT3B in living cells. It has been designed to recognize 5-HT3B from rat and mouse samples. The antibody is unlikely to recognize the receptor from human samples.

    Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032)

General Protocols

  • Blocking Peptides – Controls for better results
  • Blocking Peptide Protocol for Western Blot (WB)
Peptide Blocking Overview Diagram

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