Overview
Type: Synthetic peptide
Form: Lyophilized powder
5HT3B Receptor/HTR3B (extracellular) Blocking Peptide (#BLP-SR032) is the original antigen used for immunization during Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032) generation. The blocking peptide binds and ‘blocks’ Anti-5HT3B Receptor/HTR3B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):1,2. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032), (1:200).
3,4. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody, preincubated with 5HT3B Receptor/HTR3B (extracellular) Blocking Peptide (#BLP-SR032).
Expression of Serotonin receptor 3B in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. A. 5-HT3B staining (green) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counter stain. C. Merged image of A and B.
Properties
Sequence
- (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B (Accession Q9JJ16).
Accession (Uniprot) Number Q9JJ16
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
