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Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody

5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B

Cat #: ASR-032
Alternative Name 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
  • Peptide (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B (Accession Q9JJ16). Extracellular, N-terminus.
Accession (Uniprot) Number Q9JJ16
Gene ID 58963
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical.
Human- not recommended for use with human samples.
RRID AB_11124667.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.95 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):
    Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):
    1,2. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032), (1:200).
    3,4. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody, preincubated with 5HT3B Receptor/HTR3B (extracellular) Blocking Peptide (#BLP-SR032).
  • Expression of Serotonin receptor 3B in rat DRG
    Expression of Serotonin receptor 3B in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. A. 5-HT3B staining (green) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counter stain. C. Merged image of A and B.
  1. Thompson, A.J. and Lummis, S.C. (2006) Curr. Pharm. Des. 12, 365.
  2. Faerber, L. et al. (2007) Eur. J. Pharmacol. 560, 1.
  3. Ronde, P. and Nichols, R.A. (1998) J. Neurochem. 70, 1094.
  4. Morales, M. and Wang, S.D. (2002) J. Neurosci. 22, 6732.
  5. Sudweeks, S.N. et al. (2002) J. Physiol. 544, 715.
  6. Davies, P.A. et al. (1999) Nature 397, 359.
  7. Monk, S.A. et al. (2001) Neuropharmacology 41, 1023.
Scientific background

5-Hydroxytryptamine receptor 3B (5-HT3B, HTR3B) belongs to the superfamily of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the superfamily of G-protein coupled receptors.

The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.

In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The localization of 5-HT3B subunits is somewhat controversial; some studies show that its expression is restricted to the peripheral nervous system and others show that it is also detected in the brain and in hippocampal neurons1,4-7.

5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3B. The antibody can be used in western blot  and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5-HT3B in living cells. It has been designed to recognize 5-HT3B from rat and mouse samples. The antibody is unlikely to recognize the receptor from human samples.

For research purposes only, not for human use



Scientific Background

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