αA-Conotoxin OIVA

Alpha-A-O4a, Alpha-A-OIVA
An Antagonist of α1/β1/γ/δ (fetal muscle) nAChR
Cat #: STC-520
Alternative Name Alpha-A-O4a, Alpha-A-OIVA
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Conus obscures (Obscure cone, Conus halitropus).
    Source Synthetic peptide
    MW: 1848 Da.
    Purity: >99% (HPLC)
    Effective concentration 100 - 250 nM.
    Sequence CCGVXNAACHXCVCKNTC.
    Modifications Disulfide bonds between Cys1-Cys9, Cys2-Cys14 and Cys12-Cys18. X=Hydroxyproline. Cys18 - C-terminal amidation.
    Molecular formula C70H110N24O23S6.
    Activity αA-Conotoxin OIVA blocks α1/β1/g/d (fetal muscle) nAChR1.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • Alomone Labs αA-Conotoxin-OIVA inhibits the muscle (α1/β1/δ/γ) nAChR channels heterologously expressed in Xenopus oocytes.
        A. Time course of αA-Conotoxin OIVA (#STC-520) action on α1/β1/δ/γ nAChR channels. Current amplitudes were plotted as a function of time. Membrane potential was held at –80 mV and oocytes were stimulated by exposure to solution containing 100 µM ACh + 3 µM PNU-120596 every 50 seconds. Muscle nAChR currents are inhibited by 2.5 min application of 100 nM (green) and 250 nM (red) (as indicated). B. Superimposed traces of α1/β1/δ/γ nAChR channel current in the absence (control) and presence of 100 nM (green) and 250 nM αA-Conotoxin OIVA (red) (taken from the experiment in A).
      • αA-Conotoxin OIVA (αA-OIVA) is a peptidyl toxin originally isolated from Conus obscures and belongs to the family of short αA-conotoxins (αAS) which includes αA-OIVA, αA-OIVB, αA-PeIVA, and αA-PeIVB. αA-Conotoxin OIVA blocks the fetal muscular subtype nicotinic acetylcholine receptor (α1/β1/γ/δ subunits) (IC50 = 0.51 nM) with 600-fold greater affinity than the adult subtype (IC50 = 310 nM). It completely blocks the elicited currents and it dissociates very slowly from the fetal muscle receptor. This toxin causes an apparently irreversible paralysis upon intramuscular injection into gold fish.1
    Target α1/β1/γ/δ nAChR
    Net Peptide Content: 100%
    Last update: 14/03/2019

    αA-Conotoxin OIVA (#STC-520) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use