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Azemiopsin

An Antagonist of Muscular α1/β1/γ/δ nAChR

Cat #: STA-100
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2544 Da
    Purity: >98% (HPLC)
    Effective concentration 0.5-10 µM.
    Sequence DNWWPKPPHQGPRPPRPRPKP.
    Structure
    Molecular formula C118H174N38O26.
    Activity Azemiopsin blocks the adult form (α1/β1/ε/δ) as well as the fetal form (α1/β1/γ/δ) nAChR1.
    References-Activity
    1. Utkin, Y.N. et al. (2012) J. Biol. Chem. 287, 27079.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Azemiopsin inhibits fetal muscle nAChR (α1/β1/γ/δ) heterologously expressed in Xenopus oocytes.
      Alomone Labs Azemiopsin inhibits fetal muscle nAChR (α1/β1/γ/δ) heterologously expressed in Xenopus oocytes.
      Time course of Azemiopsin (#STA-100) action on fetal muscle α1/β1/γ/δ nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at -60 mV and oocytes were stimulated by exposure to 100 µM acetylcholine every 200 seconds. 1 µM (for 5 min) Azemiopsin was perfused during the period marked by the bar.
    References - Scientific background
    1. Utkin, Y.N. et al. (2012) J. Biol. Chem. 287, 27079.
    Scientific background

    Azemiopsin is a 21 amino acid peptidyl toxin, isolated from the Azemiops feae viper venom1 and is a synthetic version of the peptide. Unlike similar venom isolates, it does not contain cysteine residues1.

    Azemiopsin efficiently competes with α-Bungarotoxin (#B-100) for binding to Torpedo nAChR and with lower efficiency to human α7 nAChR. It dose-dependently blocks ACh-induced currents in Xenopus oocytes heterologously expressing human muscle-type nAChR and is more potent against the adult form than the fetal form1. The peptide has no effect on GABA(A) receptors or on 5-HT3 receptors1.

    Azemiopsin resembles Waglerin-1 (#STW-001) (a disulfide-containing peptide from the Tropidechis wagleri venom) in its biological activity.

    Target α1/β1/γ/δ nAChR
    Peptide Content: 100%
    Last update: 29/08/2021

    Azemiopsin (#STA-100) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Shipping and Ordering information