Overview
Type: Synthetic peptide
Form: Lyophilized powder
ABCB4/MDR3 Blocking Peptide (#BLP-MT023) is the original antigen used for immunization during Anti-ABCB4/MDR3 Antibody (#AMT-023) generation. The blocking peptide binds and ‘blocks’ Anti-ABCB4/MDR3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4):1,2. Anti-ABCB4/MDR3 Antibody (#AMT-023), (1:200).
3,4. Anti-ABCB4/MDR3 Antibody, preincubated with ABCB4/MDR3 Blocking Peptide (#BLP-MT023).
Properties
Sequence
- (C)KARIFRNSTKKSLK, corresponding to amino acid residues 658-671 of rat ABCB4/MDR3 (Accession Q08201).
Accession (Uniprot) Number Q08201
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
