ADAM10 (extracellular) Blocking Peptide (#BLP-NR156) is the original antigen used for immunization during Anti-ADAM10 (extracellular) Antibody (#ANR-156) generation. The blocking peptide binds and ‘blocks’ Anti-ADAM10 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- Western blot analysis of rat spleen lysate (lanes 1 and 3) and mouse spleen lysate (lanes 2 and 4):
- Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human K562 erythroleukemia cell line lysate (lanes 2 and 4):
- Expression of ADAM10 in mouse parietal cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ADAM10 (extracellular) Antibody (#ANR-156), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ADAM10 immunoreactivity (green) appears in neuronal profiles (horizontal arrows). B. Pre-incubation of the antibody with ADAM10 (extracellular) Blocking Peptide (BLP-NR156), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Peptide CTPGESKNLGQKENG, corresponding to amino acid residues 400 - 414 of mouse ADAM10 (Accession O35598).