Alpha-Synuclein Blocking Peptide (#BLP-PZ035) is the original antigen used for immunization during Anti-Alpha-Synuclein Antibody (#APZ-035) generation. The blocking peptide binds and ‘blocks’ Anti-Alpha-Synuclein primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
- Expression of Alpha-Synuclein in rat hippocampus.Expression of Alpha-Synuclein in rat hippocampus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Alpha-Synuclein Antibody (#APZ-035-GP), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. Alpha-Synuclein immunoreactivity (red) appears in the mossy fiber terminal region (MFT). B. Pre-incubation of the antibody with Alpha-Synuclein Blocking Peptide (BLP-PZ035), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer.
- (C)KDQMGKGEEGYPQE, corresponding to amino acid residues 97-110 of rat α-synuclein (Accession P37377).