Overview
Type: Synthetic peptide
Form: Lyophilized powder
Alpha-Synuclein Blocking Peptide (#BLP-PZ035) is the original antigen used for immunization during Anti-Alpha-Synuclein Antibody (#APZ-035) generation. The blocking peptide binds and ‘blocks’ Anti-Alpha-Synuclein primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Alpha-Synuclein Antibody (#APZ-035), (1:400).
3,4. Anti-Alpha-Synuclein Antibody, preincubated with Alpha-Synuclein Blocking Peptide (#BLP-PZ035).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-Alpha-Synuclein Antibody (#APZ-035-GP), (1:400).
3-4. Guinea Pig Anti-Alpha-Synuclein Antibody, preincubated with Alpha-Synuclein Blocking Peptide (BLP-PZ035).
Expression of Alpha-Synuclein in rat hippocampus.Expression of Alpha-Synuclein in rat hippocampus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Alpha-Synuclein Antibody (#APZ-035-GP), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. Alpha-Synuclein immunoreactivity (red) appears in the mossy fiber terminal region (MFT). B. Pre-incubation of the antibody with Alpha-Synuclein Blocking Peptide (BLP-PZ035), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer.
Properties
Sequence
- (C)KDQMGKGEEGYPQE, corresponding to amino acid residues 97-110 of rat α-synuclein (Accession P37377).
Accession (Uniprot) Number P37377
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
