Anisomycin

flagecidin
Protein Synthesis Inhibitor
    Cat #: A-520
    Sizes: 5 mg, 10 mg, 25 mg, 50 mg, 100 mg
  • Lyophilized Powder
  • Bioassay Tested
  • Shipped at Room Temp.
  • Origin Isolated from Streptomyces griseolus.
    Source: Natural
    MW: 265.3 Da.
    Purity >98%.
    Effective concentration 0.1-10 µM.
    Structure
    Chemical name (2R,3S,4S)-2-[(4-Methoxyphenyl)methyl]-3,4-pyrrolidinediol 3-acetate.
    Molecular formula C14H19NO4.
    CAS number 22862-76-6.
    Activity

    Anisomycin is an antibiotic which inhibits protein synthesis1. It also activates stress-activated protein kinases (SAPK) independently from its ability to block translation.

    Storage before reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Reconstitution DMSO or Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage after reconstitution

    Up to four weeks at 4°C or three months at -20°C.
    Protect from light.

    Our bioassay
    Alomone Labs Anisomycin induces p38 SAPK phosphorylation in C6 glioma cells.
    Cells were grown to 70% confluence and then serum starved for 3 h and then stimulated with various concentrations of Anisomycin (#A-520) for 60 min. Cell proteins were resolved by SDS-PAGE and probed with antiphospho-p38 MAPK (upper panel) or with anti-p38 (lower panel) antibodies.
    References
    1. Sobin, B. A. et al. (1954) J. Am. Chem. Soc. 76, 4053.
    2. Jimיnez, A. et al. (1979) p. 1-19. In F. E. Hahn (ed.), Antibiotics, vol. 5 part 2. Springer-Verlag, New York, N.Y.
    3. Barbacid, M. et al. (1975) J. Mol. Biol93, 449.
    4. Kochi, S.K. et al. (1993) Exp. Cell. Res208, 296.
    5. Condorelli, G. et al. (2002) J. Biol. Chem. 277, 11013.
    6. Cano, E. et al. (1994) Mol. Cell. Biol14, 7352.
    7. Cano, E. et al. (1995) J. Cell Sci108, 3599.
    8. Chen, D. et al. (1996) J. Biol. Chem271, 6328.
    9. Ishikawa, Y. and Kitmura, M. (1999) Biochem. Biophys. Res. Commun. 264, 696.
    10. Bogoyevitch, M.A. et al. (1995) J. Biol. Chem270, 29710.
    11. Sanchez, et al. (1994) Nature 372, 794.
    12. Dיrijard, B. et al. (1994) Cell 76, 1025.
    13. Hibi, M. et al. (1993) Genes Dev7, 2135.
    14. Barros, L.F. et al. (1998) Molecular and Cellular Biology 18, 1844.
    15. Hemi, R. et al. (2002) J. Biol. Chem277, 8961.
    Last update: 25/12/2017

    Anisomycin is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis, by binding to 60S ribosomal subunits and blocking peptide bond formation, thereby preventing elongation and causing polysome stabilization. This action of Anisomycin monitored the cells for chromosomal DNA degradation and apoptosis.1-5

    Anisomycin has been widely used as an extremely potent activator of kinase cascades in mammalian cells, especially the stress-activated protein kinase (SAPK2/p38MAPK), kinase subtypes and p46/54JNK. 6-13

    Synergizes with growth factors and phorbol esters, anisomycin superinduce c-fos and c-jun by a number mechanisms, one of which is its ability to act as a potent signalling agonist, producing strong, prolonged activation of the same nuclear responses as epidermal growth factor or tetradecanoyl phorbol acetate.14

    Anisomycin stimulated serine phosphorylation of IRS-1 and IRS-2, reduced their ability to interact with the insulin receptor and by that blocked the insulin-induced tyrosine phosphorylation of IRS proteins.15

    Alomone Labs is pleased to offer Anisomycin (#A-520), isolated and purified from Streptomyces griseolus.

    For research purposes only, not for human use