Anti-Calcitonin Receptor (extracellular) Antibody

CT receptor, CT-R, C1A/C1B, Calcr
    Cat #: ACR-053
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide (C)HLVEVVPNGDLVRRD, corresponding to amino acid residues 201-215 of rat Calcitonin Receptor (Accession P32214).
    Accession (Uniprot) Number 116506
    Gene ID P32214
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 14/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1, 2. Anti-Calcitonin Receptor (extracellular) Antibody (#ACR-053), (1:200).
    3, 4. Anti-Calcitonin Receptor (extracellular) Antibody, preincubated with the control antigen.
    Live cell imaging / Immunocytochemistry
    Expression of Calcitonin Receptor in rat PC12 cells
    Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A, C. Extracellular staining of cells using Anti-Calcitonin Receptor (extracellular) Antibody, (#ACR-053), (1:50). B, D. Merged images of A and C, each with its respective live view of the cells.
    References
    1. Segovia-Silvestre, T. et al. (2011) BMC Res. Notes 4, 407.
    2. Barwell, J. et al. (2012) Br. J. Pharmacol. 166, 51.
    3. Taboulet, J. et al. (1998) Hum. Mol. Genet. 7, 2129.
    4. Gorn, A.H. et al. (1995) J. Clin. Invest. 95, 2680.
    5. Gorn, A.H. et al. (1992) J. Clin. Invest. 90, 1726.
    6. Gooi, J.H. et al. (2014) J. Endocrinol. 221, 181.
    Scientific background

    Calcitonin receptor (CTR) is a class B G-protein coupled receptor (GPCR) that signals via activation of cAMP and protein kinase C (PKC). It is one of 15 human class B GPCRs1,2. Apart from CTR, calcitonin receptor-like receptor (CTLR) is the only other B-class GPCR to bind calcitonin.  To date, there are three known human CTR mRNA isoforms arising from alternative splicing3.

    Calcitonin itself is a peptide hormone that contributes to bone calcification by direct inhibition of osteoclasts which resorb the bony tissue. It is part of a six-member family that has varied functionalities. By binding to receptor activity modifying proteins (RAMPs), CTR and CTLR can form receptors to every member of the calcitonin family. For instance, RAMP1 and CTR construct the calcitonin gene-related peptide (CGRP) receptor.

    Both CTR and CTLR have seven transmembrane domains, an intracellular C-terminal, and an extracellular N-terminal. To activate the receptors, their N-terminal must first capture the C-terminal of the ligand and then carried to the transmembrane loops where it bestows a conformational change upon them.

    Besides the bone, CTR has prominent roles in the kidney, CNS, placenta, ovaries, sperm, lung, and in the brain4,5.
    In osteoclasts, the expression of CTRs declines with age. Interestingly, polymorphism of the CTR gene is strongly associated with decreased fracture-risk in post-menopausal women3,6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CTR. Anti-Calcitonin Receptor Antibody (#ACR-053) can be used in western blot and live cell imaging applications. It has been designed to recognize CTR from human, rat and mouse samples.

    For research purposes only, not for human use