Anti-Adenylate Cyclase Type III (extracellular)-ATTO-488 Antibody

AC3, Adenylate cyclase type 3, ATP pyrophosphate-lyase 3, Adenylate cyclase, olfactive type, Adenylyl cyclase 3
    Cat #: AAR-043-AG
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat Adenylate cyclase type III (Accession P21932). 3rd extracellular loop.
    Accession (Uniprot) Number P21932
    Gene ID 64508
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-043), and immunocytochemistry (labeled antibody).
    Applications: ic, lci
    May also work in: fc, ih
    Live cell imaging / Immunocytochemistry
    Expression of Adenylate Cyclase Type III in rat U-87 MG cells
    Immunocytochemical staining of intact living rat U-87 MG cells. A. Extracellular staining of cells using Anti-Adenylate Cyclase Type III (extracellular)-ATTO-488 Antibody (#AAR-043-AG), (1:25), (green). B. Merge of A with live view of the cells.
    References
    1. Choi. E.J. et al. (1992) Biochemistry 31, 6492.
    2. Chern, Y. et al. (2000) Cell. Signal. 12, 195.
    3. Yang, B.  et al. (1999) Biochem. Biophys. Res. Commun. 254, 548.
    4. Seed, A.M. et al. (2012) Islets 4, 343.
    5. Hong, S.H. et al. (2013) Oncotarget 4, 1791.
    6. Wong, S.T. et al. (2000) Neuron 27, 487.
    Scientific background

    Adenylyl cyclases (ACs) are a family of enzymes that synthesize cAMP, upon stimulation. cAMP is an important second messenger, which regulates carbohydrate, lipid, protein, and nucleic acid metabolism as well as synaptic transmission, ion channel function, and transcription in neurons1. There are nine closely related isoforms of ACs (AC1–9) in mammals. All AC isoforms share a primary structure consisting of two hydrophobic regions which comprise six transmembrane helices and three large cytoplasmic domains. The basic catalytic unit of a membrane-bound AC molecule consists of the C1a and C2 domains. The N-terminus domains of ACs are highly variable, and might play a regulatory role2.

    Adenylyl Cyclase type 3 (AC3) is widely expressed in the olfactory tissue, human cell line HEK-293, bovine brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung and retina3.
    Several studies have suggested that AC3 may play an important role in the regulation of glucose metabolism and body weight. It was found that overexpression of AC3 mRNA is involved in pathophysiological metabolic processes, such as type 2 diabetes4. In addition, a recent study demonstrated the association of AC3 with gastric cancer as well as its tumorigenic potentials5.

    Furthermore, AC3-knockout mice showed peripheral and behavioral anosmia6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 22/10/2018

    Anti-Adenylate Cyclase Type III (extracellular) Antibody (#AAR-043) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and thus, is ideal for detecting AC3 in living cells. It has been designed to recognize AC3 from human, rat and mouse samples.

    Anti-Adenylate Cyclase Type III (extracellular)-ATTO-488 Antibody (#AAR-043-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Adenylate Cyclase Type III (extracellular)-ATTO-488 Antibody has been tested in live cell imaging applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
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