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Home › Products › Ion Channels › Ca2+ Channels › Voltage-Gated Ca2+ Channels › Antibodies to CaV Channels

Certificate of Analysis

Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody

Voltage-dependent calcium channel subunit alpha-2/delta-3

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Overview

Cat #: ACC-103
Alternative Name Voltage-dependent calcium channel subunit alpha-2/delta-3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CSWWHSDMTAKAQK, corresponding to amino acid residues 942-955 of rat CaVα2δ3 (Accession Q8CFG5). Extracellular.
Accession (Uniprot) Number Q8CFG5
Gene ID 306243
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2340901.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Rat brain and human chronic myelogeneous leukemia (K562) cell line lysates (1:200).
  • Western blot analysis of rat brain (lanes 1 and 3) and human K562 chronic myelogeneous leukemia (lanes 2 and 4) lysates:
    Western blot analysis of rat brain (lanes 1 and 3) and human K562 chronic myelogeneous leukemia (lanes 2 and 4) lysates:
    1,2. Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103), (1:200).
    3,4. Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody, preincubated with CACNA2D3/Cavα2δ3 (extracellular) Blocking Peptide (#BLP-CC103).
Immunohistochemistry
  • Rat brain.
Live cell imaging / Immunocytochemistry
  • Rat PC12 cells (1:25).
Scientific background

Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. On the basis of their voltage activation properties, CaV channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels2. HVA channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3. The Ca2+ channel α2δ subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield α2 and δ subunits linked by a disulfide bond with a single transmembrane segment4.  The α2δ subunit regulates many functional aspects of Ca2+ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane5.

There are four proteins that comprise CaVα2δ: CaVα2δ1, CaVα2δ2, CaVα2δ3 and CaVα2δ46. The CaVα2δ3 subunit is predominantly expressed in neuronal tissue. The CaVα2δ3 subunit regulates all classes of HVA calcium channels. The Caα2δ3 subunits in the nerve terminal function in synaptic morphogenesis and cytoskeletal organization, and that this role is independent of their function in α1 subunit localization and physiology.

CaVα2δ3 is likely to be the primary presynaptic α2δ isoform mediating morphological development of the neuromuscular junction (NMJ), since null alleles have such a large effect on NMJ development and abolish all action-potential evoked transmission7. Recent study shows that methylation-dependent transcriptional silencing of CaVα2δ3 may contribute to the metastatic phenotype of breast cancer8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
For research purposes only, not for human use
Last Update: 10/04/2023

Specifications

Citations

Citations

Applications

Scientific Background

Specific Control Product

  • CACNA2D3/Cavα2δ3 (extracellular) Blocking Peptide (#BLP-CC103) is the original antigen used for immunization during Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103) generation. The blocking peptide binds and ‘blocks’ Anti-CACNA2D3/Cavα2δ3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

    CACNA2D3/Cavα2δ3 (extracellular) Blocking Peptide (#BLP-CC103)

Related Products

Antibodies

  1. Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody (#ACC-015)
  2. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102)
  3. Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104)
  4. Anti-CACNA2D4 (CaVα2δ4) (extracellular)-ATTO Fluor-594 Antibody (#ACC-104-AR)

Explorer kits & Research packs

Explorer kits
  1. L-Type CaV Channel Antibody Explorer Kit (#AK-215)
  2. Non-L-Type CaV Channel Antibody Explorer Kit (#AK-216)

Resources

  • L-Type (CaV1) Channels
  • N-Type (CaV2.2) Channels
  • P/Q-Type (CaV2.1) Channels

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Resources

  • L-Type (CaV1) Channels

  • N-Type (CaV2.2) Channels

  • P/Q-Type (CaV2.1) Channels

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