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Anti-CLC-7 (CLCN7) Antibody

Chloride channel 7, H+/Cl- exchange transporter 7
Cat #: ACL-008
Alternative Name Chloride channel 7, H+/Cl- exchange transporter 7
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)KDLARYRLGKGGLE, corresponding to amino acid residues 783-796 of rat CLC-7 (Accession P51799). Intracellular, C-terminal.
Accession (Uniprot) Number P51799
Gene ID 29233
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 13/14 amino acid residues identical.
RRID AB_2756562.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of mouse kidney (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates:
    Western blot analysis of mouse kidney (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates:
    1,2. Anti-CLC-7 (CLCN7) Antibody (#ACL-008), (1:200).
    3,4. Anti-CLC-7 (CLCN7) Antibody, preincubated with CLC-7/CLCN7 Blocking Peptide (#BLP-CL008).
References
  1. Stolting, G. et al. (2014) Front. Physiol. 5, 378.
  2. Zhao, Q. et al. (2009) Biochem. Biophys. Res. Commun. 384, 277.
Scientific background

Chloride channels (CLCs) are a family of nine anion channels and anion/proton exchangers. While CLC-3 through CLC-7 are anion/proton exchangers, other members of the family seem to function only as anion channels. CLC exchangers, including CLC-7, are mainly expressed in intracellular compartments such as endosomes and lysosomes and are involved in housekeeping regulation of these organelles.

The CLC-7 protein is assembled as a dimer with each subunit consisting of 18 transmembrane helices followed by a cytosolic carboxy-terminus that contains two conserved cystathionine-β-synthase (CBS) domains. Three separate chloride binding sites within each subunit were identified in three-dimensional structures, Sint, Scen, and Sext, named after their position relative to the extra- or intra-cellular side of the plasma membrane. The central position forms a significant part of the selectivity filter with the conserved amino acids S107, I109, F357, and Y445 in addition to the less well conserved F348 and I356 coordinating the chloride ion within the conduction pathway1.

CLC-7 is vital for the proper function of osteoclast cells. The neutralizing current through CLC-7 is required for an efficient acidification of the cell’s resorption lacuna. CLC-7 can be inserted into this specialized plasma membrane domain by an exocytotic insertion from late endosomal–lysosomal membranes. Mice deficient of CLC-7 protein show severe osteoporosis and retinal degeneration2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CLC-7. Anti-CLC-7 (CLCN7) Antibody (#ACL-008) can be used in western blot analysis. It has been designed to recognize CLC-7 from human, mouse and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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