Guinea pig Anti-D1 Dopamine Receptor Antibody

DRD1, D(1A) dopamine receptor
    Cat #: AGP-100
    Alternative Name DRD1, D(1A) dopamine receptor
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Guinea pig
    Reactivity: h, m, r
    Immunogen
    • Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901). Intracellular, C-terminus.
    • Guinea pig Anti-D1 Dopamine Receptor Antibody
    Accession (Uniprot) Number P18901
    Gene ID 24316
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.
    RRID AB_2756622.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Guinea pig total IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Guinea pig Anti-D1 Dopamine Receptor Antibody
      Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):
      1,2. Guinea pig Anti-D1 Dopamine Receptor Antibody (#AGP-100), (1:400).
      3,4. Guinea pig Anti-D1 Dopamine Receptor Antibody, preincubated with the negative control antigen.
    • Guinea pig Anti-D1 Dopamine Receptor Antibody
      Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:

      1. Guinea pig Anti-D1 Dopamine Receptor Antibody (#AGP-100), (1:400).
      2. Guinea pig Anti-D1 Dopamine Receptor Antibody, preincubated with the negative control antigen.

    Immunohistochemistry
    • Guinea pig Anti-D1 Dopamine Receptor Antibody
      Expression of DRD1 in rat cortex
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-D1 Dopamine Receptor Antibody (#AGP-100), (1:300), followed by goat-anti-guinea pig-Cy3 antibody. DRD1 staining (red) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).
    References
    1. Missale, C. et al. (1998) Physiol. Rev. 78, 189.
    2. Zeng, C. et al. (2008) Am. J. Physiol. 294, H551.
    Scientific background

    The D1 dopamine receptor (D1 receptor, DRD1) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and Schizophrenia1.

    All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.

    Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes)1.

    The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity1.

    The D1 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex and striatum. In the periphery the D1 receptor has been detected in the adrenal cortex, kidney and heart.

    Functionally, the D1 receptor has been implicated in the regulation of both locomotor and cognitive functions including the maintaining of spontaneous motor behaviors, the control of working memory and cognition as well as the regulation of craving and reward pathways.

    In addition, the D1 receptor plays an important role in the pathogenesis of hypertension by regulating epithelial Na+ transport and by interacting with vasoactive hormones/humoral factors, such as aldosterone and angiotensin1,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat D1 Receptor. Guinea pig Anti-D1 Dopamine Receptor Antibody (#AGP-100) raised in guinea pigs can be used in western blot and immunohistochemistry applications. It has been designed to recognize DRD1 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-D1 Dopamine Receptor Antibody (#ADR-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use
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