Overview
Type: Synthetic peptide
Form: Lyophilized powder
D1 Dopamine Receptor Blocking Peptide (#BLP-DR001) is the original antigen used for immunization during Anti-D1 Dopamine Receptor Antibody (#ADR-001) generation. The blocking peptide binds and ‘blocks’ Anti-D1 Dopamine Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:200).
3,4. Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).
Expression of DRD1 in rat cortexImmunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:100), (green). A. DRD1 appears in the soma and dendrites of cortical pyramidal neurons in layer 5. B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of DRD1 is restricted to pyramidal neurons. DAPI is used as the counterstain.
Expression of DRD1 in rat striatumImmunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:100), (green). A. DRD1 appears in the striatal matrix (star). B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of DRD1 is restricted to the matrix. DAPI is used as the counterstain.
Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):1,2. Guinea pig Anti-D1 Dopamine Receptor Antibody (#ADR-001-GP), (1:400).
3,4. Guinea pig Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1. Guinea pig Anti-D1 Dopamine Receptor Antibody (#ADR-001-GP), (1:400).
2. Guinea pig Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).
Expression of DRD1 in rat cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-D1 Dopamine Receptor Antibody (#ADR-001-GP), (1:300), followed by goat-anti-guinea pig-Cy3 antibody. DRD1 staining (red) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901).
Accession (Uniprot) Number P18901
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
