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Anti-D1 Dopamine Receptor Antibody

DRD1, D(1A) dopamine receptor

Cat #: ADR-001
Alternative Name DRD1, D(1A) dopamine receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901). Intracellular, C-terminus.
Accession (Uniprot) Number P18901
Gene ID 24316
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.
RRID AB_2039826.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:200).
    3,4. Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).
Immunohistochemistry
  • Expression of DRD1 in rat cortex
    Expression of DRD1 in rat cortex
    Immunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:100), (green). A. DRD1 appears in the soma and dendrites of cortical pyramidal neurons in layer 5. B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of DRD1 is restricted to pyramidal neurons. DAPI is used as the counterstain.
  • Expression of DRD1 in rat striatum
    Expression of DRD1 in rat striatum
    Immunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:100), (green). A. DRD1 appears in the striatal matrix (star). B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of DRD1 is restricted to the matrix. DAPI is used as the counterstain.
References
  1. Missale, C. et al. (1998) Physiol. Rev. 78, 189.
  2. Zeng, C. et al. (2008) Am. J. Physiol. 294, H551.
Scientific background

The D1 dopamine receptor (D1 receptor, DRD1) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and Schizophrenia1.

All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.

Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes)1.

The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity1.

The D1 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex and striatum. In the periphery the D1 receptor has been detected in the adrenal cortex, kidney and heart.

Functionally, the D1 receptor has been implicated in the regulation of both locomotor and cognitive functions including the maintaining of spontaneous motor behaviors, the control of working memory and cognition as well as the regulation of craving and reward pathways.

In addition, the D1 receptor plays an important role in the pathogenesis of hypertension by regulating epithelial Na+ transport and by interacting with vasoactive hormones/humoral factors, such as aldosterone and angiotensin1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-D1 Dopamine Receptor Antibody (#ADR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DRD1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat colon lysate.
    Li, Y. et al. (2019) Am. J. Physiol. 316, C393.
Immunohistochemistry citations
  1. Mouse artery sections.
    Crockett, S.L. et al. (2019) Pediatr. Res. 87, 991.
  2. Rat colon sections.
    Li, Y. et al. (2019) Am. J. Physiol. 316, C393.
  3. Rat brain sections (1:100).
    Yang, Y.L. et al. (2019) Front. Neurosci. 13, 195.
Immunofluorescence citations
  1. Mouse artery sections.
    Crockett, S.L. et al. (2019) Pediatr. Res. 87, 991.
More product citations
  1. Cai, Q.Q. et al. (2013) Auton. Neurosci. (2013) 176, 48.
  2. Fiorentini, C. et al. (2013) Neurobiol. Dis. 54, 339.
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