Anti-GPR43/FFAR2 Antibody

G-protein coupled receptor 43, Free fatty acid receptor 2
    Cat #: AFR-032
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    Immunogen
    Peptide (C)RGAEETVEGTKTDR, corresponding to amino acid residues 301-314 of rat FFAR2 (Accession Q76EI6). Intracellular, C-terminus.
    Accession (Uniprot) Number Q76EI6
    Gene ID 292794
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse -12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Will not recognize human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip
    Western blot
    Western blot analysis of rat stomach (lanes 1 and 3) and small intestine (lanes 2 and 4) lysates:
    1,2. Anti-GPR43/FFAR2 Antibody (#AFR-032), (1:500).
    3,4. Anti-GPR43/FFAR2 Antibody, preincubated with the control peptide antigen.
    Western blot analysis of mouse colon lysate:
    1. Anti-GPR43/FFAR2 Antibody (#AFR-032), (1:200).
    2. Anti-GPR43/FFAR2 Antibody, preincubated with the control peptide antigen.
    References
    1. Stoddart, L.A. et al. (2008) Pharmacol. Rev. 60, 405.
    2. Bindels, L.B. et al. (2013) Trends Pharmacol. Sci. 23, 226.
    Scientific background

    Fatty acids have long been recognized for the variety of their effects in the body. Yet, until recently, these actions were thought to be mediated exclusively via actions on cellular metabolism. There are currently three known receptors for free fatty acids: FFA1, FFA2, and FFA3.

    Free fatty acid receptor 2 (FFA2) is a G-protein coupled receptor containing seven hydrophobic regions, consistent with transmembrane (TM) spanning helices. FFA2 contains cysteine residues in the first and second extracellular loops that are likely to contribute to structure via the formation of intramolecular disulfide bonds. FFA2 mRNA is detected in a number of tissues, but the highest expression is found in immune cells such as neutrophils, monocytes, peripheral blood mononuclear cells, B-lymphocytes, and polymorphonuclear cells.

    Short chain fatty acids (SCFAs) are fatty acids of less than six carbons and were found to be endogenous agonists of FFA2. The most potent agonist of the receptor is acetate (C2) and propionate (C3) being equipotent followed by butyrate (C4) and then valerate (C5) and formate (C1)1.

    FFA2 is implicated in the pathogenesis of several diseases. Stimulation of FFA2 by acetate allows resolution of a colitis-related inflammatory response. FFA2 knockout mice show exacerbated or unresolved inflammation in cases of acute and chronic colitis, arthritis, and asthma. In contrast, other studies show that knockout mice exhibit an increased mortality compared to control mice, despite reduced immune cell recruitment, decreased colonic inflammation, and attenuated colonic tissue damage2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/12/2018

    Anti-GPR43/FFAR2 Antibody (#AFR-032) is a highly selective antibody directed against an epitope of the rat Free fatty acid receptor 2. The antibody can be used in western blot analysis. It has been designed to recognize FFAR2 from mouse and rat samples. The antibody is not suited to recognize FFAR2 from human samples.

    For research purposes only, not for human use