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Anti-GPR40/FFAR1 (extracellular) Antibody

G-protein coupled receptor 40, Free fatty acid receptor 1

Cat #: AFR-011
Alternative Name G-protein coupled receptor 40, Free fatty acid receptor 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
May also work in: h*, m*
  • Peptide, (C)NVASFINPDLEGSWRK corresponding to amino acid residues 244-259 of rat FFAR1 (Accession Q8K3T4).
Accession (Uniprot) Number Q8K3T4
Gene ID 266607
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 15/16 amino acid residues identical; human - 12/16 amino acid residues identical.
RRID AB_2340936.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat pancreas lysate: 
    Western blot analysis of rat pancreas lysate: 
    1. Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011), (1:200).
    2. Anti-GPR40/FFAR1 (extracellular) Antibody, preincubated with GPR40/FFAR1 (extracellular) Blocking Peptide (#BLP-FR011).
  • Expression of GPR40/FFAR1 in rat brain
    Expression of GPR40/FFAR1 in rat brain
    Immunohistochemical staining of rat brain sections using Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011). A. GPR40 immunoreactivity (green) in the cerebellum appears in the molecular layer (MOL) and in Purkinje cells (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
Indirect flow cytometry
  • Cell surface detection of GPR40/FFAR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of GPR40/FFAR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC..
    ___ Cells + Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011), (2.5μg) + goat-anti-rabbit-FITC.
  1. Stoddart, L.A. et al. (2008) Pharmacol. Rev. 60, 405.
  2. Briscoe, C.P. et al. (2003) J. Biol. Chem. 278, 11303.
  3. Itoh, Y. et al. (2003) Nature 422, 173.
  4. Brown, A.J. et al. (2003) J. Biol. Chem. 278, 11312.
  5. Le Poul, E. et al. (2003) J. Biol. Chem. 278, 25481.
  6. Karaki, S. et al. (2006) Cell. Tissue Res. 324, 353.
Scientific background

Free fatty acids (FFAs) were for long believed to influence cellular metabolism. Hoever, the discovery that FFAs actually mediate their effects through one of three G-protein coupled receptors (GPCRs) - Free Fatty Acid Receptors 1-3 (FFAR1-3), has initiated a number of studies in order to assess their implication in health and disease1.

Like all other GPCRs, FFAR1-3 have seven transmembrane domains, an extracellular N-terminal tail and an intracellular C-terminus.

FFAR1 is activated by either medium or long, saturated or unsaturated fatty acids2,3, whereas FFAR2 and FFAR3 are activated by short chain fatty acids4,5. FFAR1 is coupled to Gq and leads to Ca2+ mobilization upon activation. FFAR2 couples to both pertussis-sensitive and insensitive G-proteins and also leads to increased intracellular Ca2+ levels. FFAR3 coupling to G-protein induces an increase in cAMP1.

Distribution studies of all three FFA Receptors indicate that FFAR1 mRNA is quite elevated in the pancreas2,3. FFAR1 is also detected in the liver, heart, skeletal muscle, and brain; although this broad localization pattern is supported only by one study2. FFAR2 is expressed in many tissues like liver and colon6. FFAR3 is expressed in many tissues but shows highest expression in adipose tissue4. Relatively high expression of the receptor could be detected in pancreas, spleen, lymph nodes and bone marrow4,5.

As mentioned above, the finding that FFAs’ actions are mediated by GPCRs, prompted many studies in order to elucidate their roles in healthy and disease states. Such studies stipulate that FFAR1 may be involved in obesity and type 2 diabetes since circulating free fatty acid levels in the plasma are significantly elevated. On the other hand, FFAR2 and FFAR3 could be potential targets for treating irritable bowel disease and appetite control1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011) is a highly specific antibody directed against an epitope of the rat Free fatty acid receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize FFAR1 from mouse, rat, and human samples.

For research purposes only, not for human use
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