- Peptide (C)DQSANEKNKLEMNK, corresponding to amino acid residues 345-358 of rat GABRB1 (Accession P15431). 2nd intracellular loop.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-GABA(A) β1 Receptor Antibody (#AGA-010), (1:500).
3,4. Anti-GABA(A) β1 Receptor Antibody, preincubated with GABA(A) β1 Receptor Blocking Peptide (#BLP-GA010).
- Bonnert, T.P. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 9891.
- Korpi, E.R. et al. (2002) Prog. Neurobiol. 67, 113.
- Sieghart W. (2006) Adv. Pharmacol. 54, 231.
- Russek, S.J. (1999) Gene 227, 213.
- Russek, S.J. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 8600.
- Kertes, D.A. et al. (2011) Alcohol. Clin. Exp. Res. 35, 496.
- Parsian, A. et al. (1999) Am. J. Med. Genet. 88, 533.
γ-Aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the vertebrate central nervous system (CNS) mediates fast synaptic transmission via ionotropic GABA(A) receptors. These receptors are pentameric assemblies of individual subunits arranged around a central, chloride-conducting pore. To date, 16 human GABA(A) receptor subunit genes have been characterized and grouped together according to their amino acid similarity and termed: α(1–6), β(1–3), δ(1–3), γ, ε, θ, and π1. Each subunit has a large extracellular N-terminal domain, four hydrophobic membrane-spanning domains that form the channel region and a small extracellular C-terminus2. These subunits can combine in many different ways to form functional receptors, but most synaptic receptors contain two α subunits (α 1–3 isoforms), two β subunits, and a γ subunit arranged in the order γ-β-α-β-α3.
The β1 subunit gene is located in the β1- α4- α2- γ1 gene cluster on chromosome 4, and is most highly expressed in the adult rat hippocampus4. GABA(A) R genes are postulated to play a role in neuronal maturation, synaptogenesis, and predisposition to neurological disease. Increases in GABA levels and changes in GABA(A) R subunit gene expression, including decreased β1 mRNA levels, have been observed in animal models of epilepsy5. Recent genetic studies have implicated GABA(A) β1 Receptor (GABRB1) in bipolar disorder, schizoaffective disorder and major depression6. In addition, GABRB1 is associated with the risk of alcohol dependence7.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat GABA(A) β1 receptor. Anti-GABA(A) β1 Receptor Antibody (#AGA-010) can be used in western blot analysis. It has been designed to recognize GABRB1 from rat, mouse and human samples.
- Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001)
- Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003)
- Anti-GABA(A) β3 Receptor Antibody (#AGA-013)
- Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005)
- Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018)
- Anti-GABA(A) π Receptor (extracellular) Antibody (#AGA-019)
- Anti-GABA(A) ρ1 Receptor (GABRR1) (extracellular) Antibody (#AGA-006)