Anti-GPR126/ADGRG6 (extracellular) Antibody

Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR
  • New
Cat #: AGR-056
Alternative Name Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KDPVQIKIKHTRTQE, corresponding to amino acid residues 753 - 767 of mouse GPR126 (Accession Q86SQ4). Extracellular, N-terminus.
    • Anti-GPR126/ADGRG6 (extracellular) Antibody
    Accession (Uniprot) Number Q86SQ4
    Gene ID 57211
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*, lci*
    Western blot
    • Anti-GPR126/ADGRG6 (extracellular) Antibody
      Western blot analysis of mouse lung lysate (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):
      1, 2. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
      3, 4. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-GPR126/ADGRG6 (extracellular) Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
      2. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-GPR126/ADGRG6 (extracellular) Antibody
      Western blot analysis of human LNCaP prostate carcinoma cell line lysate (lanes 1 and 5), human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 6), human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human K562 erythroleukemia cell line lysate (lanes 4 and 8):
      1-4. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
      5-8. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with negative control antigen.
    References
    1. Langenhan, T. et al. (2013) Sci. Signal. 6, 276.
    2. Sun, P. et al. (2020) Sci. Adv. 6, 12.
    3. Küffer, A. et al. (2016) Nature 536, 464.
    4. Paavola, K.J. et al. (2014) Sci. Signal. 7, ra76.
    Scientific background

    GPR126 is an adhesion G-protein coupled receptor; the second largest subgroup of GPCRs1. The defining characteristic of the adhesion GPCR family is their extraordinarily large N-terminal extracellular domain important to communicate with extracellular ligands and mediate adhesive functions2.

    GPR126 is implicated in an increasing number of developmental defects: skeletal defects, including shortened height, adolescent idiopathic scoliosis, arthrogryposis multiplex congenital, and periodontitis. Other studies reveal that GPR126 is essential for myelination of axons in the peripheral nervous system and the heart2.

    Known ligands of GPCR126 are type IV collagen (COLIV) in peripheral nerves and inner ear development, Laminin-211 during Schwann cell development, and prion protein in myelination3, suggesting that GPR126 could use different signaling pathways in different tissues4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 15/06/2020

    Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize ADGR6 from human, rat, and mouse samples.

    For research purposes only, not for human use
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