Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody

Calcium-independent alpha-latrotoxin receptor 1, CIRL-1, Adhesion G protein-coupled receptor L1, ADGRL1, Lectomedin-2, LEC2
    Cat #: ALR-021
    Alternative Name Calcium-independent alpha-latrotoxin receptor 1, CIRL-1, Adhesion G protein-coupled receptor L1, ADGRL1, Lectomedin-2, LEC2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide CEPREVRRVQWPATQ(G), corresponding to amino acid residues 480-494 of rat Latrophilin-1 (Accession O88917). Extracellular, N-terminus.
        Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody
    Accession (Uniprot) Number O88917
    Gene ID 65096
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_11219339.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
      • Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
        1,2. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (1:200).
        3,4. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody
        Expression of Latrophilin-1 receptor in rat hippocampus
        Immunohistochemical staining of rat hippocampal dentate granule layer (G) using Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021). A. Latrophilin-1 receptor staining (red) appears in astrocytes. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
      • Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody
        Expression of Latrophilin-1 receptor in SH-SY5Y cell line
        Cell surface detection of Latrophilin-1 receptor in intact living human neuroblastoma (SH-SY5Y) cells. A. Extracellular staining of cells with Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (red), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI is used as the counterstain (blue). C. Merged images of A and B.
    References
      • Latrophilins (Latrophilin1-3) are members of the adhesion G-protein coupled receptor subfamily. Like all GPCRs, Latrophilins have seven transmembrane domains and are distinguished by a large extracellular N-terminal tail and a large intracellular C-terminal tail1. The N-terminus has several cell adhesion domains and undergoes proteolysis after synthesis, while the C-terminal has various consensus post-translational sites like phosphorylation and palmitoylation2. In addition, Latrophilins undergo alternative splicing3.

        Latrophilin-1 was discovered by its ability to bind α-Latrotoxin (α-LTX), a toxin isolated from the black widow spider venom4. α-LTX induces exocytosis by creating a Ca2+ influx in the presynaptic membrane. α-LTX can also stimulate small vesicle exocytosis in a Ca2+ independent manner. Three receptors have been found to bind α-LTX. Of the three, Latrophilins are responsible for the Ca2+-independent effects of α-LTX2. The binding of α-LTX to Latrophilin-1 increases exocytosis of neurotransmitters5,6.

        In an attempt to find the natural ligand of Latrophilin-1, Lasso, a splice variant of Teneneurin-2 was discovered to be an endogenous binding partner of the adhesion-GPCR7. Teneneurins are large glycoproteins with a single transmembrane domain8,9. Like Latrophilin-1, Teneneurins are mostly expressed in the brain where they modulate neurite outgrowth, axon guidance and synaptogenesis7.

        Regarding the localization of Latrophilins, Latrophilin-1 is expressed predominantly in the brain, Latrophilin-2 is highly expressed in the liver and lung, while Latrophilin-3 is almost exclusively detected in the brain1,10.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 03/12/2019

    Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Latrophilin-1 from rat, human, and mouse samples.

    For research purposes only, not for human use
    Citations
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