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Anti-GSG1L Antibody

Germ cell-specific gene 1-like protein, GSG1-like protein
Cat #: AGC-048
Alternative Name Germ cell-specific gene 1-like protein, GSG1-like protein
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CRHERYPTRHQPH, corresponding to amino acid residues 283-295 of rat GSG1L (Accession D3ZK93). Intracellular, C-terminus.
Accession (Uniprot) Number D3ZK93
Gene ID 499263
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 12/13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1, 2. Anti-GSG1L Antibody (#AGC-048), (1:200).
    3, 4. Anti-GSG1L Antibody, preincubated with GSG1L Blocking Peptide (#BLP-GC048).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Anti-GSG1L Antibody (#AGC-048), (1:200).
    2. Anti-GSG1L Antibody, preincubated with GSG1L Blocking Peptide (#BLP-GC048).
Immunohistochemistry
  • Expression of GSG1L in rat hippocampus
    Expression of GSG1L in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GSG1L Antibody (#AGC-048), (1:200), followed by goat anti-rabbit-AlexaFluor-488. GSG1L immunoreactivity (green) appears in the outer molecular layer (OML) and in the granule layer (GL) of the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).
References
  1. Twomey, E.C. et al. (2017) Neuron 94, 569.
  2. Gu, X. et al. (2016) Nat. Commun. 7, 10873.
  3. Azumaya, C.M. et al. (2017) PLoS One 12, e0174742.
Scientific background

Germline-specific gene 1-like (GSG1L) is a tetra-spanning membrane protein which belongs to the Claudin protein superfamily. It acts as an auxiliary subunit that modulates the assembly, trafficking and gating of AMPA receptors (AMPAR)1.

GSG1L has four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm, the juxtamembrane region in its C-tail extracellular Loop1 domain is critical for its suppression of AMPAR-mediated synaptic transmission2.

AMPA receptors are ligand gated ion channels critical for synaptic transmission and plasticity. Their dysfunction is involved in a variety of psychiatric and neurological diseases ranging from major depressive disorder to amyotrophic lateral sclerosis. A newly explored strategy is to manipulate the activity of AMPA receptors is through compounds that affect specific AMPAR-auxiliary subunit complexes. Overexpression of GSG1L strongly suppresses AMPAR, and GSG1L knockout enhances AMPAR-mediated synaptic transmission which makes it a leading therapeutic candidate for manipulating AMPAR activity3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-GSG1L Antibody (#AGC-048) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GSG1L from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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