GSG1L Blocking Peptide (#BLP-GC048) is the original antigen used for immunization during Anti-GSG1L Antibody (#AGC-048) generation. The blocking peptide binds and ‘blocks’ Anti-GSG1L primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of GSG1L in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GSG1L Antibody (#AGC-048), (1:200), followed by goat anti-rabbit-AlexaFluor-488. GSG1L immunoreactivity (green) appears in the outer molecular layer (OML) and in the granule layer (GL) of the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).
- CRHERYPTRHQPH, corresponding to amino acid residues 283 - 295 of rat GSG1L (Accession D3ZK93).