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Anti-Homer3 Antibody

Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3
Cat #: APZ-028
Alternative Name Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RSQSADAPGPTERER, corresponding to amino acid residues 155-169 of rat Homer3 (Accession Q9Z2X5). Intracellular.
Accession (Uniprot) Number Q9Z2X5
Gene ID 29548
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human – identical; mouse – 14/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1,2. Anti-Homer3 Antibody (#APZ-028), (1:200).
    3,4. Anti-Homer3 Antibody, preincubated with Homer3 Blocking Peptide (#BLP-PZ028).
  • Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line (lanes 2 and 4) lysates:
    Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line (lanes 2 and 4) lysates:
    1,2. Anti-Homer3 Antibody (#APZ-028), (1:200).
    3,4. Anti-Homer3 Antibody, preincubated with Homer3 Blocking Peptide (#BLP-PZ028).
Immunohistochemistry
  • Expression of Homer3 in rat nucleus basalis
    Expression of Homer3 in rat nucleus basalis
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Homer3 Antibody (#APZ-028), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. Homer3 immunoreactivity (green) appears in cells of the horizontal diagonal band (HDB), (arrows). Cell nuclei are stained with DAPI (blue).
References
  1. Xiao, B. et al. (1998) Neuron 21, 707.
  2. Shiraishi-Yamaguchi, Y. and Furuichi, T. (2007) Genome Biol. 8, 206.
  3. Soloviev, M.M. et al. (2000) J. Mol. Biol. 295, 1185.
  4. Wu, J. et al. (2015) Mol. Biol. Cell 26, 1629.
  5. Li, Z. et al. (2013) Asian Pac. J. Cancer Prev. 14, 2525.
Scientific background

The HOMER protein family includes Homer1, 2, and 3. These proteins are mainly expressed in the neuronal post-synaptic density (PSD) where they play a role in post-synaptic signaling and receptor trafficking by forming multivalent links with various receptors and PSD scaffolding proteins. The expression level of Homer proteins in non-neural tissues is very low in comparison with that in the nervous system, HOMER3 also expressed in the lung, spleen, kidney and ovary. In the mouse brain HOMER3 is concentrated in the dendritic spines of Purkinje cells connecting the parallel fibers and is also present in their axons, In the hippocampus, HOMER3 is concentrated in the CA2-CA3 regions1,2.

All three proteins in the HOMER family have several isoforms as a result of alternative splicing, HOMER3 are shown to exist in both long and short forms, the latter without the C-terminal coiled-coil domains. HOMER3 mRNA is subject to alternative splicing at five different sites giving rise to ten variants of HOMER3 proteins. HOMER1b and HOMER3 have been shown to form a tetramer via the carboxy-terminal domain3.

HOMER3 directly binds to type I metabotropic glutamate receptors, which act via phospholipase C to stimulate IP3-mediated release of Ca2+ from intracellular vesicles3.

HOMER proteins have also been implicated in axon guidance during brain development4.

HOMER3 can regulate transcription and play a very important role in the differentiation and development for some tissues such as muscle and the nervous system. Studies show that HOMER3 abnormal expression plays a role in acute myeloid leukemia (AML). Heterologous expression of HOMER3 in K562 cells inhibits proliferation, influences the cell cycle profile, affects apoptosis and also promotes cell differentiation5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Homer3 Antibody (#APZ-028) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer3 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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