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Anti-Shank1 Antibody

SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon
Cat #: APZ-011
Alternative Name SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)RSQEGRQESRSDKAK, corresponding to amino acid residues 614-628 of rat Shank1 (Accession Q9WV48). Intracellular, between the SH3 and PDZ domains.
Accession (Uniprot) Number Q9WV48
Gene ID 78957
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/15 amino acid residues identical.
RRID AB_2341061.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity This antibody will recognize all known rat isoforms of Shank1 with the exception of isoform 2 (see Q9WV48-2).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
    1,2. Anti-Shank1 Antibody (#APZ-011), (1:400).
    3,4. Anti-Shank1 Antibody, preincubated with Shank1 Blocking Peptide (#BLP-PZ011).
Immunohistochemistry
  • Expression of Shank1 in rat hippocampus
    Expression of Shank1 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Shank1 Antibody (#APZ-011), (1:300), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Shank1 staining (green) is detected in interneurons (vertical arrows) and in the pyramidal layer (P, horizontal arrows). Nuclei are stained with DAPI (blue).
References
  1. Jiang, Y. H. and Ehlers, M.D. (2013) Neuron 78, 8.
  2. Sheng, M. and Kim, E. (2000) J. Cell Sci. 113, 1851.
  3. Stella, S.L. Jr. et al. (2012) PLoS One 7, e43463.
  4. Guilmatre, A. et al. (2014) Dev. Neurobiol. 74, 113.
  5. Lim, S. et al. (1999) J. Biol. Chem. 274, 29510.
  6. Lennertz, L. et al. (2012) Eur. Arch. Psychiatry Clin. Neurosci. 262, 117.
  7. Hung, A.Y. et al. (2008) J. Neurosci. 28, 1697.
Scientific background

Shank family proteins (shank1, shank2 and shank3) are neuronal scaffold proteins that organize a cytoskeleton-associated signaling complex at the postsynaptic density (PSD) of nearly all excitatory glutamatergic synapses in the mammalian brain1.

The Shank family, including Shank1, has five conserved protein domains: 1. ankyrin repeat domain (ANK), 2. Src homology 3 (SH3) domain, 3. PSD-95/Discs large/ZO-1 (PDZ) domain, 4. proline-rich region containing homer and cortactin-binding sites (Pro), 5. sterile α motif (SAM) domain2. All these domains can act as sites for specific protein-protein interactions, and the presence of these multiple domains within a single polypeptide is consistent with a function as a scaffolding protein. Shanks interact directly or indirectly with all major types of glutamate receptors: NMDA receptors, AMPA receptors, and mGluRs via different domains. Shank proteins also interact with several actin regulatory proteins and L-type Ca2+ (CaV1.3) channels in medium spiny neurons of the striatum3.

Shank1 mRNA, being synthesized in dendritic spine, can locally control the spine morphology. The Shank1 gene is located on chromosome 19q13.33 and contains 23 exons and two alternative promoters leading to two distinct isoforms4. Shank1 is highly expressed in the cortex and hippocampus5.

Recent studies indicate that Shank1 is a causative gene for idiopathic autism spectrum disorders (ASD)1. In addition it was found that Shank1 has a role in working memory deficits in schizophrenia6. Shank1 knock-out mice display increased anxiety and decreased vocal communication7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Shank1. Anti-Shank1 Antibody (#APZ-011) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Shank1 from human, rat, and mouse samples.

For research purposes only, not for human use
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