Anti-Human CD97/ADGRE5 (extracellular) Antibody

Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation
  • New
Cat #: AER-055
Alternative Name Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    • Peptide (C)KSDSDRGGHWATEG, corresponding to amino acid residues 499-512 of human CD97/ADGRE5 (Accession P48960). Extracellular, N-terminus.
    • Anti-Human CD97/ADGRE5 (extracellular) Antibody
    Accession (Uniprot) Number P48960
    Gene ID 976
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity The antibody will not recognize the protein from rat or mouse samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-Human CD97/ADGRE5 (extracellular) Antibody
      Western blot analysis of HepG2 (lanes 1 and 5), Jurkat (lane 2 and 6), K562 (lanes 3 and 7) and THP-1 (lanes 4 and 8) cell lines lysates:
      1-4. Anti-Human CD97/ADGRE5 (extracellular) Antibody (#AER-055), (1:200).
      5-8. Anti-Human CD97/ADGRE5 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-Human CD97/ADGRE5 (extracellular) Antibody
      Cell surface detection of CD97/ADGRE5 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Human CD97/ADGRE5 (extracellular) Antibody (#AER-055) (2.5 µg/5x105 cells) + goat-anti-rabbit-FITC.
    1. Hamann, J. et al. (1996) Genomics 15, 144.
    2. Zhao, W. et al. (2017) Mol. Med. Rep. 15, 3048.
    3. Leemans, J.C. et al. (2004) J. Immunol. 172, 1125.
    4. Spendlove, I and Sutavani, R. (2010) Adv. Exp. Med. Biol. 706, 138.
    5. Hsiao, C.C. et al. (2011) FEBS Lett. 585, 313.
    Scientific background

    CD97, encoded by the adhesion G protein-coupled receptor 5 (ADGRE5) gene, is a member of the epidermal growth factor (EGF) seven transmembrane receptor family, belonging to the adhesion family of G protein-coupled receptors (GPCRs)1. CD97 is widely expressed on the surface of lymphoid cells, macrophages, smooth muscle cells and several types of tumor cells and acts through binding of decay accelerating factor (CD55) and dermatan sulfate2.

    In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation3. Antibodies against CD97 have been demonstrated to diminish various inflammatory disorders by depleting granulocytes and the interaction between CD97 and its ligand CD55 regulates T-cell activation and increases proliferation and cytokine production4.

    Changes in the expression of CD97 have been described in auto-inflammatory diseases, such as rheumatoid arthritis and multiple sclerosis. Outside the immune system, CD97 is likely involved in cell-cell interactions and ectopic CD97 expression leading to enhanced cell-cell aggregation5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 24/01/2020

    Anti-Human CD97/ADGRE5 (extracellular) Antibody (#AER-055) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CD97 from human samples only. The antibody will not recognize the protein from rat or mouse samples.

    For research purposes only, not for human use
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