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Anti-Human CXCR1 (extracellular) Antibody

C-X-C chemokine receptor type 1, Interleukin-8 receptor A, IL-8R A, IL8RA, IL-8 receptor type 1, CD181

Cat #: ACR-011
Alternative Name C-X-C chemokine receptor type 1, Interleukin-8 receptor A, IL-8R A, IL8RA, IL-8 receptor type 1, CD181
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)SNITDPQMW DFDDLN, corresponding to amino acid residues 2-16 of human CXCR1 (Accession P25024). Extracellular, N-terminus.
Accession (Uniprot) Number P25024
Gene ID 3577
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Specific to human CXCR1.
RRID AB_10557035.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.75 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of CXCR1 in human Jurkat acute T cell leukemia cell lysate:
    Western blot analysis of CXCR1 in human Jurkat acute T cell leukemia cell lysate:
    1. Anti-Human CXCR1 (extracellular) Antibody (#ACR-011), (1:200).
    2. Anti-Human CXCR1 (extracellular) Antibody, preincubated with Human CXCR1 (extracellular) Blocking Peptide (#BLP-CR011).
Indirect flow cytometry
  • Cell surface detection of CXCR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of CXCR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Human CXCR1 (extracellular) Antibody (#ACR-011), (2.5μg) + goat-anti-rabbit-FITC.
  • The blocking peptide is not suitable for this application.
References
  1. Murphy, P.M. et al. (2000) Pharmacol. Rev. 52, 145.
Scientific background

Chemokine receptors belong to the seven transmembrane domain G-protein coupled receptor superfamily and are differentially expressed in diverse cell types. In leukocytes, chemokines coordinate development, differentiation, anatomic distribution, trafficking, and effector functions and thereby regulate innate and adaptive immune responses.

To date, 18 chemokine receptors are known and designated as CXCR1-5, CCR1-11, XCR1, and CX3CR1 based on their specific chemokine preferences.

The CXC chemokine receptors specifically bind and respond to cytokines of the CXC chemokine family. CXCR1 and CXCR2 were the first chemokine receptor subtypes to be defined. They are the only known mammalian receptors for ELR+ CXC chemokines [CXC chemokines which contain the tripeptide motif glutamic acid-leucine-arginine (ELR) N-terminal to the first cysteine].

CXCR1 and CXCR2 both bind IL-8, with high affinity; they do not bind other types of chemokines. CXCR1 and CXCR2 are the major chemokine receptors expressed on neutrophils. They appear to operate mainly in acute inflammation and innate immunity.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 31/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human CXCR1. Anti-Human CXCR1 (extracellular) Antibody (#ACR-011) can be used for western blot and indirect flow cytometry applications. It has been designed to recognize human CXCR1.

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For research purposes only, not for human use

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