Free shipping starts now, no minimum, no coupons required!

Anti-CCR7 (extracellular) Antibody

C-C chemokine receptor type 7, C-C CKR-7, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, CD197

Cat #: ACR-027
Alternative Name C-C chemokine receptor type 7, C-C CKR-7, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, CD197
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m
May also work in: r*
  • Peptide (C)DEVTDDYIGDNTTVD, corresponding to amino acid residues 26-40 of human CCR7 (Accession P32248). Extracellular, N-terminus.
Accession (Uniprot) Number P32248
Gene ID 1236
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - 14/15 amino acid residues identical.
RRID AB_11219145.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of Jurkat (lanes 1 and 4), MOLT-4 (lanes 2 and 5) and J774A.1 (lanes 3 and 6) cell lysates:
    Western blot analysis of Jurkat (lanes 1 and 4), MOLT-4 (lanes 2 and 5) and J774A.1 (lanes 3 and 6) cell lysates:
    1-3. Anti-CCR7 (extracellular) Antibody (#ACR-027), (1:200).
    4-6. Anti-CCR7 (extracellular) Antibody, preincubated with CCR7 (extracellular) Blocking Peptide (#BLP-CR027).
Indirect flow cytometry
  • Cell surface detection  of CCR7 in Jurkat living cells:
    Cell surface detection of CCR7 in Jurkat living cells:
    ___ Unstained cells.
    ___ Cells + Anti-CCR7 (extracellular) Antibody, (#ACR-027) (5-10 µg/1x106 cells) followed by goat anti-rabbit-dylight 488.
  • The blocking peptide in not suitable for this application.
  1. Jin, T. et al. (2008) Cytokine 44, 1.
  2. Murphy, P.M. (2002) Pharmacol. Rev. 54, 227.
  3. Viola, A. and Luster, A.D. (2007) Annu. Rev. Pharmacol. Toxicol. 48, 171.
  4. Cardona, A.E. et al. (2008) J. Leukoc. Biol. 84, 587.
  5. Lodowski, D.T. and Palczewski, K. (2009) Curr. Opin. HIV AIDS 4, 88.
  6. Tran, P.B. and Miller, R.J. (2003) Nat. Rev. Neurosci. 4, 444.
  7. Moser, B. and Loetscher, P. (2001) Nat. Immunol. 2, 123.
  8. Pals, S.T. et al. (2007) Blood 110, 3102.
  9. Sanchez-Sanchez, N. et al. (2006) J. Immunol. 176, 5153.
  10. Gunn, M.D. (2003) Semin. Immunol. 15, 271.
  11. Randolph, G.J. et al. (2005) Nat. Rev. Immunol. 5, 617.
Scientific background

Chemokines (CHEMOtactic cytoKINES) are an important subgroup of the inflammatory cytokine family. More than fifty chemokines are expressed in mammalian cells and are characterized by their relatively small size (~70-90 amino acids), by their conserved N-terminus and cysteine motifs. This group of proteins has been further categorized on the basis of the cysteine spacing in the motifs creating C, CC, CXC, and CX3C chemokine subfamilies1,2.

All fifty chemokines exert their effects through twenty different chemokine receptors, belonging to the superfamily of G-protein coupled receptors (GPCRs) suggesting a certain level of promiscuity among the different receptors. All chemokine receptors couple to the pertussis sensitive Gi protein leading to phospholipase C activation and adenylate cyclase inhibition3.

Chemokines were first identified by their ability to mediate leukocyte chemoattraction.  Apart from regulating the migration of leukocytes, they seem to be major players during inflammation and immunity4-6. Indeed, chemokines could also be further classified as being inflammatory as many chemokines are extensively upregulated in response to inflammation, or housekeeping important for the homeostasis of certain cell types. Inflammatory chemokines are responsible for recruiting immune cells to the inflamed region, and housekeeping chemokines, expressed in lymphoid or non-lymphoid tissues mediate the trafficking and targeting of cells7,8.

In general, chemokines and their receptors guide leukocytes to sites of infection/inflammation. However, cases of chronic inflammatory disease and tissue damage occur when there is excessive recruitment of leukocytes. They could also be involved in the pathogenesis of neurological diseases like multiple sclerosis and many inflammatory diseases like atherosclerosis and inflammatory bowel disease. Recently, chemokines and their receptors have been found to be involved in cancer metastasis, namely breast cancer1. The chemokine signaling also seems to be important for the communication between neural cells and the immune system, especially in the context of infection.

CCR7 receptor is responsible for directing dendritic cell migration to the lymph nodes where they are important for initiating the immune response. This receptor has two ligands, CCL19 and CCL21 which are expressed by stromal cells in the T cell-rich lymph node area9-11.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2021

Anti-CCR7 (extracellular) Antibody (#ACR-027) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CCR7 from mouse, rat and human samples.

For research purposes only, not for human use
Shipping and Ordering information